Journal: bioRxiv

Article Title: Therapeutic modulation of the blood-brain barrier and ischemic stroke by a bioengineered FZD 4 -selective WNT surrogate

doi: 10.1101/2022.10.13.510564

Figure Lengend Snippet: a Heat map analysis of scRNA-seq data from , displaying for each cluster SCT-normalized expression of cell type markers from Zarkada et al. (ref. ) in a Seurat-integrated dataset, containing all three of the WT, Ndp KO , and Ndp KO + L6-F4-2 experimental conditions. Concordance between Zarkada et al. markers and our scRNA-seq Seurat clusters identified Cldn5 + Mfsd2a + Spock2 + D-tip, Angpt2 + Esm1 + S-tip, Unc5b + Bmx + arterial, Ptgis high venous and Mki67 + Birc5 + proliferative endothelium in our scRNA-seq dataset. The remaining cells expressing Cdh5 and Pecam1 were described as pan-endothelial (pan-EC). b Feature plots labeling expression of the indicated genes within the pan-EC, D-tip, S-tip, arterial, proliferative, and venous populations identified from ( a ) in UMAP space.

Article Snippet: RNA was quantified using TaqMan Fast Advanced Master Mix with the following probes Mm00443610_m1 Axin2 , Mm01278617_m1 Mki67 , Mm01192208_m1 Mfsd2a , Mm00550265_m1 Lef1 (ThermoFisher, 4331182).

Techniques: Expressing, Labeling

Journal: American Journal of Physiology - Endocrinology and Metabolism

Article Title: Skeletal inflammation and attenuation of Wnt signaling, Wnt ligand expression, and bone formation in atherosclerotic ApoE-null mice

doi: 10.1152/ajpendo.00501.2015

Figure Lengend Snippet: TaqMan reagents used for quantification of mRNA by qPCR

Article Snippet: Transcript levels were calculated by normalizing to the housekeeping gene mitochondrial ribosomal protein S2 using the ΔC T method ( 34 ). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 NCBI Gene Name Gene Name TaqMan # Alox12 Arachidonate 12-lipoxygenase Mm00545833_m1 Alox15 Arachidonate 15-lipoxygenase Mm01250458_m1 Axin2 Axin2 Mm00443610_m1 Mrps2 Mitochondrial ribosomal protein S2 (chob) Mm01962382_s91 Cx43 Gap junction membrane channel protein alpha 1 Mm01962382_s53 Dkk1 Dickkopf homolog 1 Mm00438422_m1 IL-1ß Interleukin-1β Mm00434228_m1 IL-6 Interleukin-6 Mm00446190_m1 Nkd2 Naked cuticle 2 homolog Mm00472240_m1 Sost Sclerostin Mm00470479_m1 Sfrp4 Secreted frizzled-related protein 4 Mm00840104_m1 TNF Tumor necrosis factor-α Mm00443258_m1 Tnfrsf11b Tumor necrosis factor receptor superfamily, member 11b (osteoprotogerin, OPG) Mm00435452_m1 Wisp1 WNT1 inducible signaling pathway protein 1 Mm00457574_m1 Wnt4 Wingless-related MMTV integration site 4 Mm00437341_m1 Wnt5a Wingless-related MMTV integration site 5A Mm00437347_m1 Wnt6 Wingless-related MMTV integration site 6 Mm00437353_m1 Wnt10b Wingless-related MMTV integration site 10B Mm00442104_m1 Wnt16 Wingless-related MMTV integration site 16 Mm00446420_m1 Open in a separate window TaqMan reagents used for quantification of mRNA by qPCR Statistics.

Techniques: Membrane